Vol. 12, Issue 12 (2023)

Author(s):
K Sailaja, BV Bhaskara Reddy, R Sarada Jayalakshmi Devi, L Prasanthi and G Mohan Naidu

Abstract:
Green gram is the most important pulse crop grown in India. Yellow mosaic infected and healthy green gram leaves were collected from Kadapa district of Andhra Pradesh during Rabi season of 2021-22. Total DNA was isolated from both diseased and healthy leaf tissue by CTAB method. The viral presence was confirmed by PCR amplification with viral specific primers. The PCR Samples were sent for sequencing and results were submitted in NCBI database. Sequence homology studies of coat protein revealed 98.3% similarity at nucleotide level and 99.6% at amino acid level with MYMIV-IN-Raichur-Rg-MF693401. Movement protein sequence shared 98% at nucleotide level and 95.2% at amino acid level with MYMIV-IN-Raichur-Bg-MT027037. The YMV-IN-Kadapa-Gg isolate shared 98.3 to 90.8% nucleotide similarity with Mungbean yellow mosaic India virus isolates from NCBI database hence it was confirmed that the begomovirus associated with Yellow mosaic disease of green gram at Kadapa district of Andhra Pradesh is MYMIV isolate.