Vol. 12, Issue 12 (2023)

Author(s):
Hemangini Chaudhari and MK Mahatma

Abstract:
In India, groundnut is cultivated in the rabi-summer period, rainy and post-rainy seasons. A short period of 10-15 days fresh seed dormancy is required in the Spanish type of groundnut to prevent in-situ seed germination in the field due to unseasonal rains at the time of crop maturity. Therefore the main aim of present study was to found different proteins which are involved in germination process during release of fresh seed dormancy. For that uniform size mature seeds were harvested and treated freshly in laboratory with 0.05% ethrel solution under sterile condition and sampled at 6 HAI (Hours after Imbibition), 12 HAI and 24 HAI, while seeds without treatment were taken as control and stored immediately after harvesting. Groundnut seed protein SDS-PAGE profiling of NRCG-14380 was carried out at four different stages. The result indicated variation in banding profiling at all four stages. These bands were extracted from gel and digested with trypsin and subjected to UPLC-MS/MS for protein identification. The result revealed identification of 64 different proteins, majority of them was identified as seed storage protein. Hence it can be concluded that seed storage proteins play immense role during dormancy release to prove energy to the developing seed during germination.