Vol. 12, Issue 12 (2023)

Author(s):
Prabhanjan Rane, Madhubala Thakre, Mahendra Kumar Verma, Jai Prakash, Chavlesh Kumar, Vartika Srivastava, PR Shashank and Niranjana Murukan

Abstract:
The standardization of pollen germination media and pollen collection methods holds significant importance in palynological studies and crop improvement. In this study, among six media combinations studied, media consisting 10% sucrose+100 mg/L boric acid+300 mg/L calcium nitrate resulted in the highest germination rate (71.6%) and could be utilized for further pollen-related studies in grapes. Notably, calcium and boron significantly influenced pollen germination. Additionally, five pollen collection methods were compared, cluster harvested at 50% flowering stage and dried for 24 hours resulted as the most effective method for grape pollen collection, striking a balance between increased pollen quantity and germination percentage (74.9%).