Vol. 12, Issue 12 (2023)

Author(s):
Suthar YM, Prajapati HN, Barad AH and Desai SG

Abstract:
In India, Citrus is one of the major grown fruit crops after banana and mango which is known for its high nutritive and refreshing value, distinct aroma, delicious taste and also for its medicinal properties. Among all the diseases that attacks citrus crops, citrus canker caused by Xanthomonas citri pv. citri is one of the most devastating disease and of great economic importance. In present study, bacteria were isolated on Nutrient agar medium from infected leaves and fruits collected from the various places of Gujarat which showed yellow, circular and mucoid colony. Purification of bacteria was done by using streak plate method. Bacteria were identified as Gram negative based on Gram’s staining. Pathogenicity test was successfully carried out using syringe inoculation method. The initial symptoms appeared on leaves in the form of lesions as dark green and later become thickened brown and corky which become raised and blister-like, growing into white or yellow spongy pustules. The pathogen was identified through Indian Type Culture Collection (ITCC), ICAR-Indian Agricultural Research Institute (IARI), New Delhi as well as by marker assisted identification using specific primers XACF and XACR. From the results of identification, the pathogen was identified as a Xanthomonas citri pv. citri.