Vol. 12, Issue 12 (2023)

Author(s):
VK Bagul, SN Hasabnis, AS Rothe and Satyam

Abstract:
In the current investigation, the pathogen causing collar rot disease was isolated from chickpea-growing regions and identified as S. rolfsii by using morphological observations. In vitro efficacy of eight plant extracts and six organic amendments were tested against the mycelial inhibition and sclerotial viability. Efficacy of plant extracts viz., L. inermis, O. tenuiflorum, A. indica, Vinca rosea, P. longifolia, M. longifolia, J. adhatoda and Z. officinale and in oil cakes Mahua, neem, groundnut, Karanja, sunflower and castor were tested. Eight plant extracts and six organic amendments were evaluated for their in vitro efficacy against sclerotial viability and mycelial inhibition. All the tested concentrations, the 20% concentration was shown to be significantly better than the 5 and 10% concentrations. All the plant extract tested, O. tenuiflorum (tulsi) at (20% conc.) found that the highest mycelium inhibition (75.12%) of pathogen under in vitro condition. All the organic amendments tested, Mahua oil cake at 20% concentration was shown to be the most effective organic amendment in terms of highest mycelial inhibition (83.70%) of pathogen under in vitro conditions. Additionally, viability of sclerotia was evaluated at incubation period of 30 minute, 8 hours, and 24 hours of incubation. All the treatment tested at 20% conc. O. tenuiflorum (tulsi) revealed that the, total inhibition of sclerotial germination (100%) at 8 and 24 hours as well as 83.33% at 30 minute of incubation. In oil cakes, the sclerotial germination was totally inhibited by mahua oil cake extracts is 100% at 18 and 24 hours and at 30 minutes, it was 84.44%.