Vol. 12, Issue 12 (2023)

Author(s):
Parita Barvaliya, Ghanshyam Patil, Arunkumar Phurailatpam, Ankit Patel, Ronak Prajapati, Mounil Mankad, Rutul Rafaliya and Bhavesh Gajera

Abstract:
The successful initiation of culture depends on the technique of surface sterilization of the explants. In the present study, an attempt was made to remove endogenous fungal contaminants from the surface of the plant material, thereby obtaining axenic cultures with the highest survival rates. Black ginger is one of the most important medicinal plants in the world. However, in micro propagation, surface sterilization is the most important step in explants preparation. The effect of different fungicides, antibiotics and disinfectants: Mercury (II) chloride (HgCl₂), sodium hypochlorite (NaOCl), hydrogen peroxide (H₂O₂) and ethanol have been evaluated to disinfect Kaempferia parviflora rhizome shoots by varying their concentrations and exposure times. Fungal contaminants formed during culture initiation were identified by DNA sequencing. In the second experiment, different nanoparticles were evaluated for their ability to disinfect rhizomes. The rate of bacterial contamination, fungal contamination, total contamination and survival rates of the culture were observed. The results showed that among all the sterilization methods, 100% AgNP for 60 min was the most effective treatment method. The highest percentage of uncontaminated cultures was achieved at 90.00% with this treatment.