Vol. 12, Issue 12 (2023)

Author(s):
Ramziya PK, Raji Kanakkaparambil, Femi Francis, Pratheesh Mankuzhy, Babitha Vazhur and Ramnath V

Abstract:
This study investigated the impact of vitamin B₁₂ and folic acid (also known as vitamin B₉ or folate) on MCF-7, a human breast cancer cell line, for 24, 48, and 72 hours. This research sought to elucidate the potential role of these methyl-group-contributing nutrients in modulating cancer cell growth to offer insights that can be utilized in the field of cancer therapy. The results consistently revealed that, at all-time points, there were no statistically significant differences in cell proliferation and viability, between the treatment and control groups. These findings strongly indicated that the introduction of vitamin B₁₂ and folic acid to MCF-7 cell cultures did not trigger changes in cell functionality in terms of proliferation and viability. The collective outcomes underscore that the administration of vitamin B₁₂ and folic acid, as outlined in this study, is unlikely to induce noteworthy alterations in the examined cellular parameters of cancer cells. These results affirm the safety and potential suitability of these vitamins as therapeutic nutrients for future applications.