Vol. 12, Issue 12 (2023)

Author(s):
Sanjai GM, N Pandeeswari and P Senthilvalavan

Abstract:
Traditional, non-biodegradable plastics have many uses, which has expanded their use and environmental buildup. In the twenty-first century, this has emerged as one of the main reasons for concern over the environment globally. One biodegradable material that shows promise for replacing traditional non-biodegradable plastics is polyhydroxybutyrate (PHB). PHB is reported to have qualities that are similar to conventional plastics. PHBs are chosen as substitutes for fossil fuels in the manufacturing of biodegradable plastics due to their rapid degradation in ambient environments. In this present study cowpea rhizosphere soil sample was taken for the isolation of bacterial organisms. Pikovskaya agar media used for the process of isolation and screening PHB producing isolate was done by Sudan black staining. Priestia sp. shows maximum PHB production under the different carbon sources condition. The accumulation of polyhydroxybutyrate (PHB) granule in cells of Priestia sp. was significantly depended on the carbon sources in the production medium. PHB extraction was carried out by chloroform digestion method. The isolated priestia sp. can be used for feasible production of PHB by using various carbon sources. Among the various carbon sources, glucose was found to be the better carbon sources investigated.