Vol. 13, Issue 3 (2024)

Author(s):
Bisma Gulzar, Ikra Manzoor, MA Mir, Syed Zainab Kashani, Bismat Un Nisa, AH Pandit and KM Bhat

Abstract:
‘GiSelA 5’ rootstock of cherry is an outcome of (Prunus cerasus x Prunus canescens L.). It bears prominent features like great cold resistance, tolerance to a modest quantity of virus, and resistance to bacterial canker. This study optimizes a reliable protocol for in vitro sterilization for propagation of ‘GiSelA 5’ clonal rootstock of cherry.In this experiment, five separate sterilisation procedures viz., 1% sodium hypochlorite (NaClO) for five minutes (S1), 10% mercuric chloride (HgCl2) for ten minutes (S2), 70% ethyl alcohol for ten seconds (S3), (S4) : (S1) + (S3) and S5: (S2) + (S3) were followed. The results depicted that maximum culture asepsis (%) was found in shoot tips (E1) sterilized with (S4) regime while explant survival (%) was obtained with (S1) regime in shoot tips (E1). This study yields valuable insights into a dependable and effective method for sterilization for producing high-quality plant material for commercial plantations of cherry.