Vol. 12, Issue 9 (2023)

Author(s):
Dr. Rajanna M, Dr. HM Jayapraksha, Akshaykumar and Nagamani A

Abstract:
An investigation was undertaken for estimation of bio-active peptides content of casein hydrolystaes using neutrase enzyme, molecular weight markers for cow, buffalo and goat milk samples, The total ash and moisture content of BAPs obtained from hydrolyzed casein protein fractions were evaluated separately. The results revealed that, buffalo milk had higher protein (4.65%) fat (6.55%) ash (0.82%) and SNF (16.92%) content as compared to cow and goat milk. Goat milk had shown significantly higher protein content (3.90%) as compared to cow milk (3.38%). Higher yield of α -casein (54.31%) was observed in cow milk than buffalo milk (48.95%) and lower yield (20.36%) was found in goat milk. The enzymatic hydrolysis significantly improved functional properties besides increased level of bio- active peptides along with improved digestibility. Thus, physico-chemical attribute study of Buffalo milk and Goat milk along with fractionation of proteins were carried out to identify molecular weight, chemical and gross composition of buffalo cow and goat milk.