Abstract:The present investigation was carried out to know the suitable substrates for cultivation of paddy straw mushroom (Volvariella volvaceae) using different agro wastes such as paddy straw, banana leaves, deenanath grass, maize stalks & leaves, paddy straw + maize stalks (1:1), paddy straw + banana leaves (1:1) and paddy straw + deenanath grass (1:1). Highest yield and biological efficiency was obtained from paddy straw followed by paddy + maize stalks substrates (1:1), paddy+ banana substrates (1:1), respectively.. Minimum yield was obtained from deenanath grass. In order to find out best supplements, five different supplements such as wheat flour, chickpea grain powder, maize flour, rice bran and rice flour were used on paddy straw Among different supplements, highest yield and biological efficiency was obtained from chickpea powder, followed by wheat flour, maize flour used on paddy straw substrate, respectively. Minimum yield was obtained from control bed.