Vol. 7, Issue 10 (2018)

Author(s):
Shelly Kapoor and Anil Handa

Abstract:
Surveys conducted in 40 peach orchards of commercial peach growing orchards in the districts of Solan, Sirmour, Shimla and Kullu in Himachal Pradesh revealed incidence of prunus necrotic ring spot virus (PNRSV). Diffused chlorotic spots, necrotic rings, mid vein distortion and shot holes were the predominant symptoms. Leaves from symptomatic trees were collected for serological detection through DAC and DAS-ELISA and DIBA. All these tests resulted in the detection of PNRSV in trees marked on the basis of visual symptoms. Association of PNRSV with viral symptoms in peach was successfully confirmed by ELISA and DIBA assays in the present studies.