Vol. 7, Issue 11 (2018)

Author(s):
Nandhini S, Mangala Gowri A, Indhumathi S, Vaishnavi AS, Meenambigai TV and Bharanidharan GR

Abstract:
Hematopoietic stem cells (HSCs) are the well characterized adult stem cell that has been accepted for autologous cell therapy after minimal manipulation. The clinical use of HSCs to treat a variety of human and animal disorders has made them a key building block in the foundation of regenerative medicine. They represent a potential source of adult stem cells for transplantation in Veterinary Medicine. In the present study, Canine peripheral blood from apparently healthy animals were subjected for progenitor cells separation using an enrichment protocol and subjected for colony formation cell assay. The standardized simple protocol for progenitor cell enrichment and CFC assay would be applicable in clinical autologous cell therapy and can be used to analyze effects of drugs and biomolecules that induce erythropoiesis in canines as a model applicable in analysis of cell based clinical therapy.