Vol. 7, Issue 11 (2018)

Author(s):
Dermisha S, Priya PM, Sheikh Moin Ahmad, Rinsha Balan and Mini M

Abstract:
Riemerella anatipestifer, causative agent of Riemerellosis, is a gram negative, non- sporulating and non-capsulated rod, which infects mainly domestic ducks. Kerala is experiencing great economic loss due to riemerellosis from 2008 onwards. It is very hard to control the infection in the field even after antibiotic therapy at right time. Resistant infection noticed among infected farms might have a link with biofilm forming capacity of the causative agent. Hence, in this study, formation of biofilm was estimated by tube (qualitative) and tissue culture plate (TCP) as quantitative method, using six representatives isolates of Riemerella anatipestifer (RA1, RA2, RA3, RA4, RA5 and RA6). It was identified that three of the isolates (RA1, RA5 and RA6) were biofilm formers and TCP method was considered as gold standard assay to detect the biofilm formation.