Vol. 7, Issue 11 (2018)

Author(s):
Binish T

Abstract:
In vitro culture techniques will certainly go in a long way in the multiplication of endemic, endangered and rare species. The development of, an efficient protocol for rapid clonal propagation is important to meet the pharmaceutical needs and conservation of valuable Ceropegia spiralis medicinal plants. Micropropagation was carried out using nodal explants and reported that MS medium fortified with BAP & KN alone forms an effective hormonal combination for shoot induction. The number of shoots reduced on further increase in the concentration. Nodal explants from is Ceropegia spiralis cultured on MS medium fortified with BAP (1.25mg/L) had a better response of axillary buds. Maximum number of shoot regeneration resulted on MS medium supplemented with BAP 1.25mg/L+KN 1.0mg/L (5.85±1.21) shoots/explants with 80% response. The best rooting response was obtained in a medium containing NAA in 1.25mg/L concentration. In this concentration, 5.71 ± 0.75 roots had developed from each explants and nearly 72 rooted shoots are obtained within a period of 120 days.