Vol. 7, Issue 11 (2018)

Author(s):
Sharma S, Sharma RK and Kaushik D

Abstract:
Present study was designed to evaluate cypermethrin induced changes in different morphometric parameters of developing chick embryo of Gallus domesticus. The protective effect of vitamin C against cypermethrin toxicity was also measured. Fertilized eggs of Gallus domesticus of BV 300 were divided into four groups i.e. control, vehicle, treatment and protective group and were kept in an incubator at 38±0.5°C with a relative humidity of 60-70% and proper ventilation. Control group have untreated eggs. The vehicle group was supplied with distilled water whereas the treatment group was tested for 25 mg/L of cypermethrin, both via immersion technique. The eggs in protective group were administered with vitamin C by air sac method. 16-day old embryos were recovered from all the groups for assessing morphometric parameters including the mortality rate and number of surviving embryos having deformities, crown ramp length, net body weight, head diameter, eye diameter, beak and limb length. Results of present study showed that the mortality rate and number of surviving embryos having deformities were significantly increased upto 27% and 68% respectively in cypermethrin exposed group as compared to control group. The crown ramp length, net body weight, head diameter showed significant decline whereas eye diameter, beak and limb length showed slight alterations in treated groups. Vitamin C supplemented group showed sign of recovery in maintaing overall morphology of developing chick embryo against cypermethrin.