Vol. 8, Issue 1 (2019)

Author(s):
Dr. C Sivaraj, V Aswitha, M Srinidhi, K Saraswathi and P Arumugam

Abstract:
Millingtonia hortensis L. (Indian cork tree) belongs to the family of Bignoniaceae. The leaves used as sinusitis, cholagogue, antipyretic and tonic in folklore medicine. The aim of present research study was to evaluate the antibacterial, antioxidant activities of methanol leaf extract of M. hortensis and to identify the bioactive compounds by GC-MS analysis. Different types of antioxidant assays were carried out for the evaluation of antioxidant activity. Antibacterial activity was carried out by well diffusion method, in which the leaf extract showed maximum zone of inhibition of 22 mm for Micrococcus luteus at 500 µg/mL concentration. The maximum DPPH˙ radical scavenging activity was 92.49±0.41 at 300 µg/mL concentration and the IC50 was 140.97 µg/mL concentration. The maximum superoxide (O2. ) radical scavenging activity was 54.30±0.13 at 60 µg/mL concentration and the IC50 was 51.53 µg/mL concentration. The maximum Fe3+ reduction and Mo6+ reduction were 72.76±0.22 and 88.85±0.28 at 120 µg/mL concentration. The RC50 of Fe3+ and Mo6+ reduction were 21.41 µg/mL and 21.45 µg/mL concentration respectively. The GCMS analysis of methanol extract of leaves of M. hortensis revealed the presence of bio-active compounds such as Isoquinoline,1-ethyl, Flavone, Oleic acid, Coumarine,8-methoxy-3-(4-nitrophenyl) and Phenol,2,6-bis (1,1-dimethylethyl)-4-[(4-hydroxy-3,5-dimethylphenyl) methyl] exhibiting various therapeutic applications such as antioxidant, antimicrobial, anti-proliferative and immuno-modulatory activities.