Vol. 8, Issue 2 (2019)

Author(s):
Priyanka, Vijay J Jadhav, Sneh Lata Chauhan and SR Garg

Abstract:
In the present study, High Performance Liquid Chromatography with Ultra-Violet detector (HPLC-UV) technique was standardized and validated for the detection and quantitation of penicillin antimicrobial residues viz. amoxicillin and cloxacillin from milk. The standardization procedure showed that the values for the system precision (% RSD) for both the analytes was <9% for area and <0.6% for retention time), linearity (r²>0.99), specificity and accuracy (70-110%) and precision (<10%) were within accepted range and demonstrated system suitability for analysis of milk samples. The standardized and validated method was applied for the detection of penicillin residues from 100 milk samples randomly collected from local market of Hisar City (Haryana). Mean concentrations of amoxicillin and cloxacillin antimicrobial residues in market milk samples were 26.15 and 1.14 µg/kg, respectively. A total of 14 samples were found to be containing penicillin antimicrobial residues. Comparison of antimicrobial concentration in each positive sample of milk with international MRLs showed that, cloxacillin was responsible for maximum violations (3%). It was concluded that milk is significant source of antimicrobial residues.