Vol. 8, Issue 2 (2019)

Author(s):
Babita Belal, Om Prakash and Viveka Nand

Abstract:
The present investigation was carried out to evaluate the altitudinal variation between two lentil (Lens culinaris Medik.) genotypes on the basis of their chemical profile, total phenolic content and antioxidant activitiy. Pulse samples were collected from two different altitudes of Uttarakhand state. The chemical profiling was done via Gas chromatography-Mass spectrometry analysis. A total of 45 and 33 phytoconstituents accounting for 76.51% and 81.03% of the total extract has been identified in the sample from lower altitude and higher altitude respectively. Antioxidant activity was determined in terms of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, hydroxyl radical scavenging and metal chelating assays. Results revealed that the genotype from higher altitude exhibits higher antioxidant potential than the genotype from lower altitude. A significant positive correlation has been observed between altitude and total phenolic content, whereas a significant negative correlation between altitude and 50% inhibitory concentration (IC₅₀) of both genotypes.