Vol. 8, Issue 4 (2019)

Author(s):
Madhuri Pradhan and Amitava Paul

Abstract:
The field experiment was conducted during post-kharif season, 2015 and pre-kharif season, 2016 at the Agricultural Farm of Palli Siksha Bhavana (Institute of Agriculture), Visva-Bharati, Sriniketan. Dry and homogeneous seeds of two varieties of sesame were irradiated with different doses of gamma rays viz. 250, 300, 350 and 400 Gy. Different types of chlorophyll mutants were identified in M₂ generation and frequency of mutants and spectrum of chlorophyll mutation was studied. The frequency of chlorophyll mutations increased with increase in the dose of mutagen. The highest frequency of chlorophyll mutation (0.54%) was induced in Tillotoma at 400 Gy treatment. Chlorina was highest in frequency in Rama, whereas, xantha was highest in frequency in Tillotoma. It was also observed that frequency of chlorina was the most frequent chlorophyll mutant followed by xantha > albina in both the genotypes. The variety Tillotoma appeared to be more sensitive towards the mutagenic treatment as compared to Rama.