Vol. 8, Issue 4 (2019)

Author(s):
Zafrul-Haq and Zahoor Ahmad Kaloo

Abstract:
Pyrus pyrifolia is an important pome fruit of family rosaceae. The development of in vitro micro-propagation protocol is the need of hour for each and every plant. In this backdrop the stem cuttings of the P. pyrifolia were induced to develop shoots in Murashige and Skoog medium supplemented with different combinations of hormones in different concentrations. The optimum results are obtained for establishment of nodal cuttings during spring season while the least results are obtained for shoot tips during winter season. The established shoots are inoculated for multiplication and the optimum results of multiplication are obtained with using two cytokinins and one auxin in the combination of 2 mgL^-1 BAP +2 mgL^-1 IBA + 1mgL^-1 Kn.