Vol. 8, Issue 4 (2019)

Author(s):
AJ Deshmukh and AN Sabalpara

Abstract:
Greengram variety seeds viz., GM-3, GM-4 and K-851 were collected and studied for the symptomatology, association and pathogenecity of seed mycoflora associated with the seeds. Four types of broad category of symptoms viz., bold healthy, bold discoloured, shrivelled and small seeds were observed and categorized primarily in all the seed lots. The observations on the specific symptoms on the broad categorized symptoms revealed the variety of symptoms viz., seeds having healthy green, apparentely green, mosaic, brown lesion, black lesion, red to dark red spot and whole brownish colour. Study on the confirmation of the fungi associated with specific symptoms revealed the association and presence of specific fungi on each type of seed viz., Colletotrichum sp. Was found associated with red to dark red spots while Alternaria sp. with black lesions and Macrophomina sp.with brown lesions. Presence of sterile unidentified fungi were associated with mixture of yellow and green coloured pattern on the greengram seeds, while a group of seed infecting mycoflora looked responsible for development of whole brownish discoloration. In all nine different fungi viz., Macrophomina phaseolina, Alternaria alternata, Colletotrichum capsici, Nigrospora spherica, Aspergillus sp., Rhizopus sp., Chaetomium sp., sterile aseptate and sterile septate fungus were found associated with the infected seeds of green gram. A single or mixtures of fungi were also proved pathogenic by causing loss in seed germination as well as seed to seedling transmission on seed inoculation method.