Vol. 8, Issue 5 (2019)

Author(s):
Dr. Khagga Bhavyasri, Masagouni Manisha and Dr. D Rambabu

Abstract:
Liquid chromatography- Tandem Mass Spectrometry (LC-MS/MS) is the most advanced hyphenated technique for the analysis of samples. It is the Liquid Chro”? Matographic techniques like HPLC or UPLC coupled with Tandem Mass Spectrometer. In Liquid Chromatography the mixtures of components of the sample gets separated according to their affinity towards both stationary phase and mobile phase and enter the Tandem Mass Spectrometer (MS/MS or MSⁿ) through an interface. Tandem mass spectrometry is the Mass Spectrometer coupled with another Mass Spectrometer. In MS/MS, the separated components from Liquid Chromatography are ionized and ions of selected molecular weight are allowed to enter collision cell where the ions get fragmented and are analyzed. This hyphenated technique offers increased selectivity where the lowest concentration of the component of the sample can be detected. It has a wide range of applications like proteomics, pharmacokinetic studies, forensic science, agrochemistry, etc.