Vol. 8, Issue 5 (2019)

Background: The plant Euphorbia tithymaloides L. or Pedilanthus tithymaloides L. is well distributed plant globally and known in India with various common names as Vilaayati-sher in Maharashtra, Naagaphani in Uttar Pradesh, Naagadaman and Naagadone in Madhya Pradesh.

Objectives: To establish HPTLC Fingerprint analysis of chloroform and petroleum ether extracts of whole shrub of Pedilanthus tithymaloides L. or Euphorbia tithymaloides L.

Materials and Methods: In the present study petroleum ether (ETP1, ETP2) and chloroform extracts (ETC1, ETC2) of Pedilanthus tithymaloides were selected for HPTLC fingerprinting. The best resolutions for both plant extracts were obtained with solvent system of toluene: ethyl acetate: formic acid in ratio of 10:3:1.WIN CATS planer chromatography manager software utilised for HPTLC fingerprinting. CAMAG Linomat 5 applicator used for sample application, CAMAG glass Twin Trough Chamber 20x10cm used for plate development and CAMAG TLC Scanner-3 with mercury remission florescence lamps used for detection of separated compounds at wavelength 254 nm and 366 nm.

Result: The result revealed presence of three (03) components in chloroform extracts (ETC1) and four (04) components in chloroform extracts (ETC2) of Pedilanthus tithymaloides L. or Euphorbia tithymaloides while petroleum ether extract (ETP1, ETP2) revealed nine (09) components.

Conclusion: HPTLC Fingerprint analysis of chloroform and petroleum ether extracts of whole plant of Pedilanthus tithymaloides L. or Euphorbia tithymaloides L. was indicator of presence of phyto constituents and expression of qualitative and quantitative measures in terms of Rf values with corresponding percentage area.