Vol. 8, Issue 6 (2019)

Author(s):
Urmi D Patel, Flora C Shah and Nayan K Jain

Abstract:
The main objective of present study was to investigate protective effect of methanolic extract of Syzygium aromaticum against hydralazine induced hepatotoxicity in vitro. Experimental study involved administration of hypertension drug hydralazine to goat liver homogenate in vitro in order to induce hepatotoxicity. Experimental protocol involved four experimental groups each containing 1ml goat liver homogenate. Group-I represented untreated control group. Group-II involved administration of Syzygium aromaticum extract (0.18mg/ml) to liver homogenate. Group-III represented toxin exposed group and involved administration of hydralazine (0.27mg/ml) to liver homogenate. Group-IV involved co-administration of hydralazine (0.27mg/ml) and Syzygium aromaticum extract (0.18mg/ml) to liver homogenate. Homogenate cultures were incubated in BOD incubator containing 5% CO₂ at 37ºC for 1-hour duration. After incubation period, experimental samples were subjected to biochemical analysis for evaluation of hydralazine induced hepatic injury and ameliorative potential of clove extract. Results revealed statistically significant decline in protein levels and activities of liver biomarkers like alkaline phosphatase, acid phosphatase, alanine aminotransferase and aspartate aminotransferase while significant elevation was observed in lipid peroxidation in hydralazine exposed group as compared to control group. Co-administration of Syzygium aromaticum extract to hydralazine exposed liver homogenate cultures maintained all studied biochemical parameters nearest to control group. In conclusion, methanolic extract of Syzygium aromaticum remarkably exhibited hepatoprotective activity and therapeutic potential against hydralazine induced toxicity.