Vol. 8, Issue 6 (2019)

Author(s):
Dushyant Yadav, Vijay Singh, Akhil Patel, SS Yadav, AK Ojha, Anuj Kumar, Sarvjeet Yadav, Brijesh Kumar, Brijesh Yadav and Atul Saxena

Abstract:
The present investigation was aimed to study the effect of Glutathione on membrane intigrity of Hariana bull spermatozoa through Hypo-Osmotic Swelling Test (HOST) during the different stages of semen preservation; after dilution, pre-freeze and post thaw, using Tris based dilutors. After the primary evaluation like progressive motility more than 70% and sperm concentration more than 600 millions spermatozoa/ml of collected ejaculates (N=24) of bulls were used for cryopreservation. The samples were extended in a Glycerolated Egg Yolk Tris (GEYT) Extender upto 80 millions sperm /ml. Addition of various concentrations 0.0 mM (Control), 0.5 mM (T1) & 1.0 mM (T2) of Glutathione was done in the diluted semen. Semen was frozen in liquid nitrogen vapour in French Mini Straw. The plasma membrane integrity of spermatozoa was evaluated through HOST. A significant difference(P<0.05) was observed between control and treatment groups for mean percentage of HOST reacted spermatozaoa after dilution of semen with the mean of 76.30±0.38, 85.00±0.41 and 79.83±0.49 percent in control, T1 and T2 groups respectively. Similar trend was aslo observed at pre-freez stage in control, T1 and T2 groups with the mean of 67.47±0.34, 77.14±0.83 and 72.72±0.48 percent respectively. The percentage of HOST reactive spermatozoa was significantly (P<0.05) higher in treatment groups at post-thaw stage with mean value of 53.15±0.61, 65.33±0.53 and 59.84±0.58 in control, T1 and T2 groups respectively. Addition of 0.05 mM (T1) Glutathione was found signinificantly different between the two treatments and more benificial in terms of membrane integrity compared to 0 mM (control) and 1.0 mM (T2) in all the stages of (after dilution, pre-freezing and following post-thaw) semen preservation. There is no any signifcant difference between the bulls in term of HOST reactive spermatozoa.