Vol. 8, Issue 9 (2019)

Author(s):
Priyanka S Bharadwaj

Abstract:
This study focuses on extraction and partial purification of peroxidase enzyme from Ipomoea batatas the sweet potato, using ammonium sulphate precipitation coupled by dialysis tubing. The activity of the extracted enzyme was determined spectrophotometrically at different stages of purification. The assay was done using Ortho-Phenylenediamine Dihydrochloride (OPD) as substrate at room temperature. OPD, in the presence of peroxidase enzyme, reacts with hydrogen peroxide, to produce 2, 3-diaminophenazine, yellow-orange compound which has absorption maxima of 437 nanometer. The concentration of the total enzyme was calculated using the Lowry’s method followed by the determination of the specific activity of the protein.