Vol. 8, Issue 5 (2019)

Author(s):
VM Wagh

Abstract:
In present study, a successful attempt has been made to develop a simple method for the simultaneous determination of Ofloxacin and Tinidazole in presence of Urine using Differential Pulse Voltammetry (DPV) technique. Quantification of Ofloxacin and Tinidazole was done in Britton-Robinson Buffer of pH 4.75 using 1M KCl as a supporting electrolyte. Both Ofloxacin and Tinidazole exhibit reduction cathodic peak in given pH with peak potential at -1.21 V for Ofloxacin and -0.25 V for Tinidazole vs SCE. 0.1N CH₃COOH was used as Solvent for the analysis. The parameters used for the method validation are LOD, linearity, assay and recovery. Proposed method was found to be simple, precise, and accurate and can be successfully applied for routine quality control analysis and simultaneous determination of Ofloxacin and Tinidazole in combined drug formulations in presence of Urine.