Vol. 8, Issue 10 (2019)

Author(s):
A Bello, P Sriram, T Ramasarmy, L Kalaiselvi and K Nagarajan

Abstract:
The demand for compounds of natural origin with health promoting effect is increasing throughout the world. Marine macroalgae remain to be a great source of bioactive compounds with potential therapeutic and nutraceutical indications. The present study aimed at evaluating the antimutagenic, anticlastogenic and DNA protective effect of methanolic extract of brown seaweed Stoechospermum marginatum using in vitro bacterial reverse mutation (Ames) test and in vivo rodent micronucleus and alkaline comet assay. In Ames test, the test extract demonstrated a moderate antimutagenic activity of about 35.20% at the highest dose of 5000 µg/plate when tested against direct and indirect-acting mutagens. Additionally, the test extract exhibited a significant anticlastogenic effect of about 27.60% and DNA protective effect of about 32.22% in a dose-dependent manner when tested at the highest dose of 2000 mg/kg body weight against cyclophosphamide-induced micronucleus in mice bone marrow erythrocytes and DNA damage in peripherally lymphocytes. The result obtained from this study indicated that methanolic extract of Stoechospermum marginatum possessed an antigenotoxic activity and can confer protective effects to living cell genome against the deleterious effects of various chemical mutagens and clastogens following exposure.