Vol. 9, Issue 2 (2020)

Author(s):
Sandeep Kumar, SP Dahiya, Ankit Magotra, Yogesh Bangar and Asha Rani Garg

Abstract:
Leptin hormone primarily expresses in the adipose tissues and has significant importance in regulating various functions like growth, puberty, reproduction and milk production in both animals and humans. The objective of the present study was to determine the polymorphism of Leptin (LEP) gene in Harnali sheep. For analyzing Leptin gene polymorphism, polymerase chain reaction- restriction fragment length polymorphism method was used. A total of 110 Harnali sheep were selected and the genomic DNA was isolated by phenol-chloroform extraction method. The PCR product (463 bp) of the Leptin gene was screened. For detection of a particular SNP in coding region of the Leptin gene, the PCR product was digested with Cail1 restriction enzyme for detection of SNP. The digested products were resolved on 2.5% agarose gel. All studied samples had only 463 bp after digestion with Cail1 restriction and resolved into monomorphic pattern BB in Harnali sheep. The results indicated highly conserved sequence of Leptin gene in Harnali sheep.