Vol. 9, Issue 3 (2020)

Author(s):
Vanita Amarsingh Khatri, Ojas Patel and Harilal Patel

Abstract:
A novel, simple and sensitive method for the determination of Orciprenaline sulphate in rat plasma using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was developed and validated. The determination was performed on an API-4500 Q-trap mass spectrometry in the multiple reaction monitoring mode using the respective [M+H] + ions m/z 212.2 / 152.1 for Orciprenaline. The lower limit of quantification was 1.000 ng/mL in rat plasma. Good linearity was obtained over the range of 1.000 to 1000 ng/mL and the correlation coefficient was found to be 0.9988. The intra and inter-day precisions were found to be 1.63 to 4.65% and 3.65 to 11.06%, respectively. The intra and inter-day accuracy derived from QC samples was found to be 96.47 to 108.97% and 99.35 to 101.779%, respectively. The analyte was stable under various conditions (at room temperature, during freeze-thaw, in wet extract conditions and under deep-freeze conditions).