Vol. 9, Issue 3 (2020)

Author(s):
Ishfaq Faried, Nishant Kumar, Rajan Sharma, Nusrat Nabi Khan and Dibyendu Chakraborty

Abstract:
TLR4 is a candidate gene for providing innate resistance towards mastitis infection. The present study was conducted to find polymorphism in exon 3 of TLR4 gene of Jersey crossbred cattle and to find association if any with somatic cell count, an indicator trait of mastitis resistance or susceptibility. Alu I RE digestion revealed three genotypic patterns AA, AB and BB with genotypic frequency of 0.28, 0.52 and 0.20 respectively. PIC value and value of heterozygosity indicate marker of moderate importance. Nei and Shannon information index suggests medium gene diversity. All the animals included in the association study showed only one genotype AB (except two) with respect to Alu I/ PCR-RFLP. Therefore, no association could be established between polymorphism of TLR-4 gene and incidences of mastitis. Using more sensitive techniques like SSCP and sequencing, exploring total sequence of TLR4 for variation and polymorphism study of other candidate genes like MHC can give more insight in host resistance towards mastitis.