Vol. 9, Issue 6 (2020)

Author(s):
Palled Mahesh Shivbasappa, Shailendra Suryawanshi Sanjay, Siddhant Mardolkar, Mahendra Kumar chauhan and Sunil Satyappa Jalalpure

Abstract:
The objective of proposed research work was to develop and validate new simple, selective, specific, accurate, precise and robust Ultra High Performance liquid chromatographic (UHPLC) method for estimation of Atenolol in bulk and pharmaceutical products. Method was developed by utilizing mobile phase composed of methanol: 0.1% orthophosphoric acid (50:50 v/v) with the use of BDS Hypersil C₁₈(250 × 4.6 mm, 5 µm) column as stationary phase. The flow rate of mobile phase was 1.5ml/min and the temperature was maintained at 20 ^oC. Atenolol was detected at 224nm using UV detector. The retention time of Atenolol was found to be 8.9 min. It showed linear response between the concentration ranges 4-8 µg/ml with correlation coefficient 0.999. The method was validated as per current ICH guidelines in terms of specificity, selectivity, linearity and range, precision, accuracy and robustness and all the values of validation was found to be within the acceptance. Hence the method can be used for the routine quality control evaluation of Atenolol in bulk powder.