Vol. 10, Issue 3 (2021)

Author(s):
Nisaath Begum, P Shivakumar, A Gopala Reddy, B Ramya, B Anil Kumar and M Usha Rani

Abstract:
The present study aimed to evaluate the protective effects of aqueous leaf extract of Tinospora cordifolia and vitamin C against cisplatin induced cardiotoxicity. A total of 36 Male Wistar rats were divided into six groups (n = 6). Group 1(Normal Control) was administered Normal saline. Group 2 was administered single dose of cisplatin @ 7.5 mg/kg body weight intraperitoneally on day 1. Groups 3 and 4 were administered for 14 days (p/o) with aqueous leaf extract of Tinospora cordifolia @ 400 mg/kg body weight and vitamin C @100 mg/kg bodyweight, respectively from day 1. Group 5 and 6 received aqueous leaf extract of Tinospora cordifolia @ 400 mg/kg body weight and vitamin C @ 100 mg/kg body weight, respectively for 14 days (p/o) along with cisplatin @ 7.5 mg/kg body weight intraperitoneally on day 1. After 14 days of experimental procedure, blood was collected and serum was separated for estimation of cardiac biomarkers; rats were euthanized and heart tissue samples were collected for histopathological examination. The biochemical assays showed a significant (P < 0.05) increase in serum LDH, CPK and troponins in group 2 when compared with group 1, and there was significant (P < 0.05) improvement in the treatment groups 5 and 6. Histopathological studies revealed degenerative changes, loss of architecture and necrosis in heart tissue of group 2 treated with cisplatin alone. These changes were reversed in groups 5 and 6. In conclusion, Tinospora cordifolia and vitamin C were found to possess protective action against cisplatin induced cardiotoxicity.