Vol. 10, Issue 4 (2021)

Author(s):
Siddique RA, Atreja SK, Khan MA, Bharti MK, Ranjan K, Garg A and Ali N

Abstract:
Acrosome reaction is very important reaction which occurs in capacitated spermatozoa and involves the fusion of outer acrosomal membrane with overlying plasma membrane and release of mainly acid hydrolases and renders the potential of fertilization in spermatozoa. Spermine-NONOate a nitric oxide donor also causes the AR through the synthesis of cGMP and activation of protein kinase G (PKG). In this study there is protein tyrosine phosphorylation of p20.p32, p45, p49, p69, p80 and p105 proteins. Spermine-NONOate in presence of PKG inhibitor resulted in significant inhinbitoon of AR as compared to spermine-NONOate alone (<0.05). Spermine-NONOate exhibited increased percentage of AR as compared to the control (<0.05). Therefore to spermine-NONOate caused the percent AR through activation of cGMP/PKG pathway.