Vol. 10, Issue 4 (2021)

Author(s):
V Thriveni, D Sharma and Zenu Jha

Abstract:
The study was conducted to investigate the effect of pre-treatments, incubation temperature and culture media in different eggplant genotypes through anther culture. The eggplants were given with different 1-4 hours of pre-treatments in combination with two different incubation temperatures 25 ^oC for 3 weeks and 35 ^oC for 8 days under dark condition. Anthers were implanted in two different C medium MS Media and N₆ supplemented with 2,4-D 2mg/l. Among different treatment combinations the treatment MS media +3 hours pre-treatment + incubation at 25 ℃ for 3weeks showed maximum embryogenic calli induction with response frequency 19.55 per cent and took least number of days for callus initiation (12 days) and lowest calli responding frequency with 1h pretreatment interaction with N6 medium supplemented with 2,4-D @ 2ml/l (0.33%). A preculture treatment of -3h at 5 ℃ exhibited the maximum calli induction frequency (12.34%) fallowed by 2.30h preculture (8.55) and minimum frequency was obtained in A1-1h preculture (0.39).