Vol. 10, Issue 4 (2021)

Author(s):
D Saiprasad, L Ramsingh, E Sunil Anand Kumar and G Ambica

Abstract:
The objective of this experiment was to study the effect of Catalase in Deccani ram semen preservation. The semen parameters like motility, viability, HOST, acrosomal integrity and sperm abnormalities were evaluated at refrigeration temperature (5 °C) after 24, 48, 72 hrs of post collection and dilution. Ten ejaculates per ram were collected by artificial vagina making a total of 60 ejaculates from six Deccani rams and extended with Egg yolk citrate. Catalase (CAT @ 100 IU) added to each ml of extended semen and control group (without any addition of antioxidant). The present experiment results reveal that addition of catalase 100 IU improved sperm cell parameters such as motility, livability, HOST and acrosomal integrity and also decreased sperm abnormalities when compared to the control group during liquid storage of semen up to 72 hrs at refrigerator temperature (5 ºC). In the present study, all semen parameters significantly (P≤ 0.05) differ between test and control groups.