Vol. 10, Issue 4 (2021)

Author(s):
Kirti Kaundal, Mohinder Kaur, Shweta Sharma, Rajneesh Thakur and Monica Sharma

Abstract:
The aim of this study is to isolate and classify novel fungi from apple replant sites in Rohru, Himachal Pradesh (Maggota and Siao). The fungal species were isolated from normal and replant sites. The isolation of fungi from leaves and fruits was implemented by inoculating (1ml) from serial dilutions (103-106) on Potato Dextrose Agar (PDA) plates. The plates were incubated at 28 ̊C for one week, then the fungal colonies were observed and pure cultures were maintained. For the molecular identification of the isolated fungi at the species level, the extracted fungal DNA was amplified by PCR using specific internal transcribed spacer primer (ITS1/ITS4). The PCR products were sequenced and compared with the other related sequences in GenBank (NCBI). Three fungal isolates from replant site of orchard at Maggota showed similarity i.e. ARF1 (Fusarium oxysporum, 99%), ARF2 (Sarocladium zeaestrain, 97%) and ARF3 (Curvularia australiensis, 99%) by ITS gene sequencing method were isolated and identified from all resources and locations.