Vol. 10, Issue 6 (2021)

Author(s):
Shiwanand Pandey, Satya Prakash, Yogesh Prasad, Arvind Kumar, Vaishali, Pooran Chand and Gopal Singh

Abstract:
An experiment was conducted at the Tissue culture Lab Department of Horticulture, Sardar Vallabhbhai Patel University of Agriculture and Technology, Modipuram, Meerut, Uttar Pradesh, India during the year 2018-20. Effect of different sterilization agent for surface sterilization under in-vitro culture of pepino with completely randomized design (CRD) having four treatment duration (1.5, 3.0, 4.5, 6.0 minutes) and used mercuric chloride (HgCl₂ 0.1%), ethanol (70%), mercuric chloride (HgCl₂ (0.1%) + ethanol (70%), sodium hypochlorite (NaOCl) (70%) and mercuric chloride (HgCl₂ 0.1%) + fungicide (Bavistin) (0.15%) for surface sterilization agent. The response of different sterilization agents was evaluated on the basis on contamination percentage at 10 days after and survived percentage at 25 days after. Minimum contamination percentage (26.35) percent and maximum survival percentage (73.13) per cent was noted under HgCl₂ (0.1%) treating for a period of 4.5 minute. Minimum contamination percentage (39.55) percent and maximum survival percentage (60.37) per cent was noted under ethanol (70%) treating for a period of 4.5 minutes. Minimum contamination percentage (31.55) percent and maximum survival percentage (68.07) per cent was noted under mercuric chloride (0.1%) + ethanol (70%) treating for a period of 4.5 minutes. Minimum contamination percentage (38.53) percent and maximum survival percentage (61.46) per cent was noted under sodium hypochlorite (NaOCl) (70%) treating for a period of 4.5 minutes. Minimum contamination percentage (21.76) percent and maximum survival percentage (77.36) per cent was noted under mercuric chloride (HgCl2 0.1%) + fungicide (bavistin) treating for a period of 4.5minutes. On the basis of results obtained present investigations, it can be concluded that contamination and survival percentage of explants was vice versa to the duration of the treatment.