Vol. 10, Issue 6 (2021)

Author(s):
K Antony Prajwala, P Subbaramamma and M Viswanath

Abstract:
Pomegranate was commercially propagated through hardwood cuttings or by air layering. However the supply of true to type planting material was scarce due to several limitations like low multiplication rate, requirement of large quantity of propagating material, rapid expansion of area under pomegranate cultivation and use of very slow propagation methods. Establishment of new plantlets require more time and are easily prone to pests and disease attack can leads to non- availability of quality plantlets through-out the year. In vitro technique has the potential to solve the problems and supply of quality planting material through-out the year. So, there was a ample scope for large scale multiplication of desired genotypes within a short time through micro propagation technique in pomegranate. In this review we had reported various steps followed in micro propagation techniquesviz., selection of explants type, effective explants sterilization methods chemicals, preservation of phenol exudation use of efficient media for culture establishment and shoot proliferation use of growth regulators and effective photoperiod etc involved in regeneration of pomegranate plantlets through in vitro culture. The hardening of plantlets by using different rooting media association with VAM fungi was also discussed.