Vol. 10, Issue 6 (2021)

Author(s):
Prakriti Meshram, Sandeep Bhandarkar, Shubha Banerjee, Ritu R Saxena, Rameshwar Prasad Sah, Mangla Parikh, DK Rana and SK Nair

Abstract:
29 polymorphic simple sequence repeat (SSR) markers were analyzed in 34 F₃ progenies derived from the cross of CG Zn Rice I and IR64 rice cultivars to investigate the association with BPH resistance. Parental polymorphism survey was taken up between donor parent IR64 and recurrent parent CG Zn Rice I. The parent DNA was isolated to good purity using the chemical method of purification. The PCR reactions were carried out according to standard protocol for rice microsatellites. A total of 68 SSR markers spanning all the 12 chromosomes of rice genome were analyzed on the susceptible variety CG Zn Rice I and resistant variety IR64 for parental polymorphism. Out of 68 SSR markers, 29 were polymorphic showing overall 42.64% polymorphism. The maximum polymorphism of 100% was observed for chromosome 9 and minimum of 16.66% for chromosome 5.Markers RM5, RM237, RM6, RM489, OSR13, RM55, RM124, RM413, RM510, RM454, RM11, RM125, RM152, RM215, RM25, RM316, RM484, RM552, RM271, RM171, RM224, RM287, RM277, RM5479, RM313, RM1986, RM3331, RM28004 and RM7102 showed polymorphism.