Vol. 10, Issue 7 (2021)

Author(s):
Manoj Kumar, Yogesh Prasad, Ajay Yadav and Amit Kumar

Abstract:
Effect of surface sterilization agents’ i.e. Sodium hypochlorite and Mercuric Chloride were tested on the contamination-free establishment of Gerbera leaf under in-vitro conditions. Sodium Hypochlorite @ 4 per cent (2, 4, 6 and 8 minutes) performed better results. Mercuric Chloride (HgCl2) @ 0.1 per cent (1, 2, 3 and 4 minutes) and the Sodium hypochlorite @ 4 per cent with Mercuric Chloride (HgCl2) @ 0.1 per cent (2, 3, 4 and 5 minutes) for time duration. Sodium hypochlorite 4 per cent (2, 4, 6 and 8 minutes) was found that the best survival percentage (41.69 %) for 8 minutes. While Mercuric Chloride (HgCl2) @ 0.1 per cent was found the best survival percentage was (62.80%) for 4 minutes However, a combination of Sodium hypochlorite 4 per cent with Mercuric Chloride (HgCl2) 0.1 per cent was found best survival percentage of (73.42%) so, the results found that the best sterilization agent was found to be a combination of mercuric chloride (HgCl2) @ 0.1 percent + Sodium hypochlorite @ 4 percent for 3 minutes.