Vol. 10, Issue 8 (2021)

Author(s):
Savankumar N Patel, VP Patel, KG Modha and VB Parekh

Abstract:
Microsatellite markers are most commonly use in fingerprinting, for assessing variation within parental lines and testing the genetic purity of hybrid seed in rice at molecular level. The present investigation was undertaken to assess the hybridity of experimental F₁ plants derived from the cross between two lines naming NVSR 2179 and NVSR 2803 having contrasting characters for yield and its attributes. Both of the parents screened using five SSR (Simple Sequence Repeat) markers to identify polymorphic marker. The SSR marker naming RM225 found to be polymorphic between two parents. Total 46 F₁s and parents were screened using polymorphic marker RM225 using PCR method. The results showed all the 46 plants are true hybrid at molecular level and can be used for development of mapping population. Screening of 100 individuals of F₂ mapping population using same SSR markers further conformed its purity base on mendalian segregation pattern of marker alleles.