Vol. 10, Issue 8 (2021)

Author(s):
Ruchi and Sashikala Beura

Abstract:
The present experiment was carried out in the laboratory of bio technology cum tissue culture centre at O.U.A.T, Bhubaneswar with the objective to obtain disease free aseptic culture of Gerbera jamesonii Bolus cv. Intense using quarter capitulum as explant. Treatments taken for surface sterilization were HgCl₂ 0.1% solution with different span of time (4, 5, 6 & 7 minutes) alone and in combination with NaOCl 0.5% solutions for 2 minutes along with control (distl. water). Lower rate of infection (6.33%), maximum percentage of asceptic cultures (93.67%) and higher survival rate (84.67%) were recorded when explants were treated with HgCl₂ 0.1% solution for 7 min. followed by NaOCl 0.5% solution for 2 min. After completion of this experiment, the quarter capitulum sterilized with the mentioned best sterilants the cultures were subjected in MS media containing different concentrations of BAP, IAA in combination for callus induction. MS media supplemented with 2.5 ppm BAP and 0.5 ppm IAA showed significantly earliness in callus induction (16.97 days), higher available days for callus proliferation (27.83 days) and maximum callus size (2.50 cm).