Vol. 10, Issue 9 (2021)

Author(s):
Sarfraz Ahmad, ML Jakhar, Varsha Kumari, DK Gothwal and HR Jat

Abstract:
Aloe barbadensis is a desert medicinal herb, immensely useful for pharmaceutical and cosmetic industries. Its micropropagation through indirect organogenesis under in vitro condition highly influences with the composition of culture media. In the present investigation, the efficacies of different types of media viz. Nitsch and Nitsch, Woody plant medium (WPM), Schenk and Hildebrant, Whites medium, Knudson Solution–C and, Murashige and Skoog (MS) medium were evaluated for indirect organogenesis. For callus induction using lateral shoot explant, media were fortified with 2,4-D (2.5 mg/l) while, a combination of 2.0 mg/l Kn + 1.0 mg/l NAA were used for de novo shoot regeneration in callus culture. Significant differences were obtained among all media compositions for all the characters studied for callus proliferation and adventitious shoot regeneration. Woody plant medium were found best for callus proliferation (3.42 g) and number of shoot regeneration (3.40). The efficacies of MS medium were also high for callusing (3.38 g) and shoot regeneration (3.30) after Woody plant medium. The rank of the different media for their relative effectiveness for callus proliferation and organogenesis can be ordered as Woody plant medium > MS medium > Nitsch and Nitsch medium > Whites medium > Knudson Solution-C = Schenk and Hildebrandt medium. These media, Woody plant medium and MS medium interchangeably can be utilized for large scale shoot multiplication of Aloe barbadensis.