Vol. 10, Issue 9 (2021)

Author(s):
Geeta Rawat, Alka Bameta and AK Gaur

Abstract:
Direct regeneration protocol has been optimized for P. kurroa using shoot tips as an explant. Shoot tips were established in culture vessels containing Murashige and Skoog (MS) basal media with solid support supplemented with different regimes of plant growth substances. Highest shooting was observed at BAP (0.5mgl^-1) and Kn(1.0mgl^-1) and this observation was extrapolated as 92.33±2.08 percentage towards shooting response based on average shoot number 24±1.73 and average shoot length 4.35±0.21(cm). Upon recording the data of rhizogenesis, highest rooting was observed at MS media supplemented with 2,4-D (0.8mgl^-1) and IAA(0.4mgl^-1). This observation data of rooting was extrapolated toward 97±3.60 percentage as a highest rooting response based on average root number 19.33±2.08 and average root length 6.1±0.36(cm). In another study, MS basal medium supplemented with different plant growth substances with different soluble Poly Vinyl Pyrrolidone (PVP) 0-1.0mgl^-1 concentration was to combat with phenolics. It was found that PVP at (0.25mgl^-1) concentration has the most suitable effect in order to control adverse effect of phenolics. This study of direct regeneration shall also pave the way for ex situ conservation of P. kurroa besides, direct regeneration also reduces the possibility of mixing of germplasm during propagation and beneficial for minimizing culture genetic make-up.