Vol. 10, Issue 9 (2021)

Author(s):
Hemant Wagh, BM Kareppa, Ravindra Satbhai, Sandesh Bangar

Abstract:
In present investigation the effect of salt induced (NaCl) stress on growth and development of callus induction of safflower variety AKS-207 was evaluated. The different concentration treatment of NaCl (50mM, 100mM, 150mM, and 200mM) with 2, 4-D (2.5mg/l) + Kinetin (1.0 mg/l) on MS media were used to screen the callus induction response. Among the four combinations of salt stress concentration 150mM NaCl with 2, 4-D (2.5mg/l) + Kinetin (1.0 mg/l) (T₄) recorded 65% growth of callus and showed hard texture and brown colour compare to other salt treatments. The control treatment (T₁) recorded white, soft and 75% callus induction response. The study revealed that the 150mM NaCl concentration was sustain the callus induction in safflower.