Vol. 10, Issue 10 (2021)

Author(s):
E Santhoshinii, N Rajinimala, R Kannan, G Ravi and R Ramamoorthy

Abstract:
Survey was conducted to record the incidence and severity of Cucumber mosaic virus disease in Cucumber in southern parts of Tamil Nadu. During Survey, the infected cucumber plants showed different symptoms viz., green and yellow mosaic pattern, dark green mottling, leaf distortion, leaf crinkling, stunting of plants, pale green with dark green warts on fruit and malformed, misshaped fruits. Cucumber plants showing the above symptoms were collected from Srivenkateshwarapuram, Chennalpatti, Vadavallanadu and Anantha Nambi kuruchchi villages of Thoothukudi district, Pallayamkottai and Thalaikulam villages of Tirunelveli district, Pavoorchatram and Vaithiyalingapuram villages of Tenkasi district and Anjugramma village of Kanyakumari district. Among the surveyed area maximum disease incidence (67.5%) and severity (3.80) were recorded in Anantha Nambi Kuruchchi, while lowest disease incidence (14.5%) and severity (0.96) were recorded in Anjugrammam. The virus inoculum was extracted from CMV infected plants by using 0.1% phosphate buffer and inoculated in cowpea and cucumber plants. Systemic symptoms viz., downward cupping, mosaic in cucumber and mosaic in cowpea were observed at 12, 21 and 23 days after inoculation respectively. Electron Microscopic observation of sap from CMV infected cowpea and cucumber plants showed 28 – 30 nm diameter size of spherical particle.