Vol. 10, Issue 11 (2021)

Author(s):
Thokchom Shitarjit Singh, OR Sathyamoorthy, Soundian Eswari, Sabiha Hayath Basha and M Parthiban

Abstract:
Adipose tissue derived mesenchymal stem cells are of considerable interest because of its easily accessible and can generate a huge proliferation of cells with minimal morbidity upon harvest. In the present study, adipose tissue samples from porcine were isolated using 0.1% collagenase type I enzyme. Cell yield was an average of 1.8 x10⁶ cells/10g of adipose tissue. Most of the adhered cells demonstrated an elongated, spindle-like fibroblastic morphology. Acridine orange/Ethidium bromide dual fluorescent staining showed no significant difference in the different passages but with increasing in the passage level, the number of viable cells decreased but increase in the number of necrotic cells.