Vol. 10, Issue 11 (2021)

Author(s):
G Raghavi, S Merina Prem Kumari, M ArumugamPillai, S Saravanan and A Kavitha Pushpam

Abstract:
The present study investigated the effect of an EMS treatment on the embryonic calli of Kavuni for the characters viz., late flowering, lodging, and long duration and improvement for these aspects will promote black rice cultivation. The experiment was conducted using Kavuni embryonic calli that showed 86.5% callus induction percentage and it was treated with EMS at 0.05%, 0.1%, 0.15% and 0.2% level along with control for mutagenesis to create dwarfism. The LC₅₀ for EMS treatment based on callus survival was 0.10%. The maximum regeneration percentage of EMS treatment was 53.6% in the control and 0.1% EMS treatment recorded 40%. The lowest plant height of 20.2 cm in 0.05% EMS treatment. Nutritional analysis recorded the maximum protein content of 5.50% at 0.05% EMS and carbohydrate content was maximum of 54.56 at 0.05% EMS. The pigment viz., β-carotene, chlorophyll and anthocyanin were maximum of 0.02 µg/g, 2.25mg/g and 1.34 mg/g respectively at 0.05% EMS treatment. Total phenolics with the highest level of 28.5 mg/g was recorded at 0.05% EMS. The in vitro mutated rice Kavuni plantlet at 0.05% EMS concentration has improved characters and selection will be made in the subsequent generation for dwarf trait.